The Early Effects of Edta, Colchicine and Azetazolamide on the Number of Osteoclasts and the Calciums in Rats

Authors

  • Tomas Hedlund
  • Anders Hulth
  • Olof Johnell

DOI:

https://doi.org/10.3109/17453678208992287

Abstract

Changes in the number of osteoclasts in rats after injection of ethylene diamine tetra-acetic acid (EDTA), azetazolamide or colchicine were studied using succinic dehydrogenase staining of osteoclasts. As early as 10 minutes after injection EDTA caused a significant increase in the number of osteoclasts. Azetazolamide and colchicine resulted in a decrease in the number of osteoclasts, apparent after 30 minutes and 60 minutes, respectively. The changes in total serum calcium after EDTA and azetazolamide administration took place at the same rate. However, azetazolamide had an effect which was the reverse of the effect of EDTA. It caused an increase in calciums in spite of a decreased number of osteoclasts. The results of this investigation confirm those of earlier studies, showing very rapid changes in the number of osteoclasts caused by substances giving rapid changes in serum calcium.

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Published

1982-01-01

How to Cite

Hedlund, T., Hulth, A., & Johnell, O. (1982). The Early Effects of Edta, Colchicine and Azetazolamide on the Number of Osteoclasts and the Calciums in Rats. Acta Orthopaedica, 53(5), 753–756. https://doi.org/10.3109/17453678208992287

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