Concentrations of proteoglycan fragments in relation to maturation, sex and time of day:Physiologic variations in knee joint fluid of rabbits

Abstract

We analyzed the concentrations of proteoglycan fragments in knee joint fluid in 142 rabbits to investigate the effect of physiologic variations—i.e., maturation, sex and time of day. The concentrations of proteoglycan fragments differed significantly between young, adolescent and adult animals and showed an inverse correlation to the stage of maturation of the rabbit. Adolescent male rabbits had higher concentrations than age-matched females. Morning and evening samples had similar concentrations. No relation was found between the proteoglycan fragment concentrations in joint fluid and the cartilage mass. The proteoglycan fragment concentrations in knee joint fluid apparently reflect the metabolic status of growing articular cartilage. There are considerable physiologic variations associated with maturation and sex, and these need to be taken into account when using the proteoglycan fragment concentration as a marker for joint diseases.